Bio-Augmentation of S2− Oxidation for a Heavily Polluted River by a Mixed Culture Microbial Consortium

The redox balance of inorganic sulfur in heavily polluted rivers might be disrupted, making reduction a major metabolic pathway sulfate-reducing bacteria (SRB), leading to massive accumulation S2− and blackening the water bodies. A mixed culture microbial consortium (MCMC) Citrobacter sp.sp1, Ochrobactrum sp.sp2, Stenotrophomonas sp.sp3 was used activate native sulfate-oxidizing (SOB) augment oxidizing process. results demonstrated that MCMC had significant oxidation effect, with 98% removal efficiency within 50 h. sulfide species varied greatly were all finally oxidized SO42−. mechanism bio-augmentation revealed through high throughput sequencing analysis. could stimulate simplify community structure cope change. microorganisms (family level) including Enterococcaceae, Flavobacteriaceae, Comamonadaceae, Methylophilaceae, Caulobacteraceae, Rhodobacteraceae, Burkholderiaceae thought associated metabolism abundance difference bio-treatment group control group. Further Pearson correlation analysis inferred functions different microorganisms: Burkholderiaceae, Alcaligenaceae, Caulobacteraceae played important roles oxidization SO42− accumulation; Campylobacteraceae, Bacteriovoracaceae, Rhodobacteraceae promoted during whole